- Oct 15, 2017
- Reaction score
- mid-Michigan, USoA
No, as little exposure to the air as possible. Making the transfer under a sterile hood and holding the cover over the vessel while the transfer is being made, with as little exposure to the air as possible. The vessel is then sealed with surgical tape to prevent contaminants from entering. The process really should be carried out under a laminar flow hood, but they are expensive. All of the instruments must be sterilized in a pressure cooker or autoclave for 22 minutes at 15 psi. It really is a very technical procedure to attempt at home with made do equipment, but it can be done. So, if you want a challenge give it a try.
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oh, no, will not be doing any more projects, i'm short on space here for much of anything new. if i bring something in i have to get rid of something else and i kinda like the stuff i have already.